Netherlands captain and goalkeeper Sari van Veenendaal has come down with a shoulder injury.
She was hurt during their opening game, a 1-1 draw with Sweden on Saturday.
Sari was replaced by Daphne van Domselaar of FC Twente after her injury.
Feyenoord goalkeeper Jacintha Weimar will join up with the squad as cover.
The 32-year-old’s absence is likely to be costly for the reigning European champions.
The Dutch captain possesses great deal of experience.
She won the 2017 Euros and reached the 2019 World Cup final with her country.
The Netherlands face Portugal on Wednesday in their second group game of Euro 2020 at Leigh Sports Village.